Czi Viewer Series Consisting Of

These delimiters can be configured in the Preferences of ImarisFileConverter or Imaris (on the Loading tab) as follows.

Imaris Stitcher initially positions images using stage coordinates stored by the microscope.

If stage coordinates are available and correct the first display of multiple images in Imaris Stitcher will already have almost good positions for each image.

The Align button in Imaris Stitcher allows to refine positions and align overlapping images precisely such that their content matches well.

Here is a typical example: If you have a file where stage coordinates are not available to ImarisStitcher you can use either a grid layout tool to position images on a grid or manually move images close to where they belong in relation to all other images.

After having done that more or less well the Align button will take care of finding precise positions.

See section File Series with Split and File Series Delimiters Configurable.

It is possible to translate and rotate the images relative to each other to facilitate alignment.

For example one may overlay a fluorescence microscopy image with an electron microscopy image of the same sample.

Most of Imaris functionality is available for each of the images when multiple images are loaded.

A few exceptions apply: Volume rendering only allows for MIP rendering in the multi-image scenario.

The Coloc module, the Easy3D viewer and the Gallery viewer are not available for multiple images.

On the other hand it is possible to calculate spots or other objects from either image.

And Imaris 9.1 will even calculate statistics of the other image when objects overlap with another image.

In the case of multiple-images multiple frame objects appear and each one can be associated with one of the loaded images.

When the position tab of the frame object is selected a little tripod appears in the 3D scene.

When the tripod is moved or rotated by clicking on the center or the axes and manipulating their position the image follows along.

This allows for efficient coarse positioning of one image relative to another in the multi-image setting.

In order to do precise positioning it can be useful to use the buttons on the position tab of the frame object to make small changes.

To facilitate positioning of the reference frame it has a little tripod that one can click with the mouse and then reposition.

The handling of mouse interactions with the tripod has been significantly improved in this version of Imaris to make positioning of the reference frame simpler than before.

Also the size of tripod is now coupled to the screen rather than to the image voxel size.

Czi Viewer Series Consisting Of

This allows to easily convert a series consisting of multiple fields of view into multiple IMS files.

If the fields of view overlap these files can then easily be opened in ImarisStitcher9.1 to be stitched into one big field of view.

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